Foundations of Methylation & Clinical Genomics

A 3-Part Practitioner Training Series with Carolyn Ledowsky

Confidently connect the dots between genetics, biochemistry, and patient outcomes.
This foundational webinar series walks you step-by-step through three of the most clinically important biochemical systems — and shows you exactly how to apply them in practice.

Whether you’re new to functional genomics or looking to strengthen your understanding, this series will help you:

Interpret genetic results with clarity.
Recognise key symptom patterns and lab markers.
Choose targeted nutrient and lifestyle strategies with confidence.

Part 1 – MTHFR & Methylation: The Essential Foundations for Clinical Practice

Unlock the key to understanding — and explaining — one of the most important genetic pathways in functional medicine.

In this first session of the series, Carolyn Ledowsky takes you step-by-step through MTHFR, folate metabolism, and methylation in a way that connects the science to what actually happens in your patient’s body — and what you can do about it.

You’ll learn:

How MTHFR polymorphisms work – C677T, A1298C, compound heterozygous variants, and what each means for enzyme function.
Why methylation is central to epigenetics – and how it impacts fertility, neurotransmitters, hormone clearance, detoxification, and immune function.
Folate pathways made clear – from food to methylfolate, the role of key enzymes like DHFR, SHMT, and MTHFD1, and why folic acid can be a hidden disruptor.
How to identify patient clues – lab markers, symptom patterns, and case history red flags that point to disturbed methylation.
Practical prescribing considerations – forms of folate and B12, cofactor nutrients, and what to do when patients “react” to methyl donors.

Clinical Takeaways:

Confidently interpret MTHFR genetic results in the context of patient presentation.
Recognise when folic acid is creating biochemical roadblocks.
Support methylation without triggering adverse reactions.
Build the biochemical foundations for the methionine cycle and downstream pathways covered in Parts 2 & 3.

Perfect for you if:
You’re a health practitioner who wants to move beyond surface-level MTHFR advice and confidently apply genetic and biochemical knowledge to real-world cases.

Part 2 – CBS & Methionine: The Critical Pathway for Detox, Neurotransmitters, and Antioxidant Defence

Move beyond the folate cycle into the central hub of methylation, detoxification, and sulphur metabolism.

In this second session, Carolyn Ledowsky breaks down the methionine cycle and the CBS (cystathionine beta synthase) pathway — the biochemical intersection where methylation, glutathione production, neurotransmitter regulation, and urea cycle function meet.
This is the pathway you’ll revisit again and again in clinical practice because it is so often at the root of complex patient presentations.

You’ll learn:

How 5-methylfolate feeds into methionine synthase and why B12 status and form matter.
Signs of CBS dysfunction – from low homocysteine and oxidative stress to sulphur sensitivity, pyroluria, and detox overload.
The oxidative stress link – why chronic inflammation diverts homocysteine away from methylation and into glutathione production.
Nutrient dependencies – how B6, zinc, magnesium, molybdenum, and amino acids influence this pathway.
The ammonia connection – how urea cycle impairments cause “brain fog,” low blood pressure, and neurological symptoms.
Hydrogen sulphide and nitric oxide interplay – their role in vascular tone, mitochondrial function, and fatigue.
Clinical red flags for CBS upregulation or downregulation – and how to approach each pattern in practice.

Clinical Takeaways:

Recognise when CBS is the “bottleneck” in your patient’s recovery.
Understand how to support glutathione production without depleting methylation.
Identify the role of the urea cycle in fatigue, cognitive impairment, and neuroinflammation.
Match B12 forms and dosing strategies to patient genetics, symptoms, and lab results.
Use targeted nutrition to restore balance in sulphur metabolism, detox pathways, and neurotransmitter function.

Perfect for you if:
You want to confidently connect the dots between methylation, detoxification, neurotransmitters, and redox balance — and know exactly where to intervene when CBS and methionine metabolism are impaired.

Part 3 – Neurotransmitters: Connecting Genetics, Mood, and Clinical Outcomes

Decode the biochemical drivers of mood, motivation, focus, and behaviour — and learn how to address them in practice.

In this advanced session, Carolyn Ledowsky unpacks the complex interplay between genetics, inflammation, and the brain’s neurotransmitter systems. You’ll discover how methylation, the biopterin pathway, and enzyme variants like MAO and COMT shape serotonin, dopamine, norepinephrine, epinephrine, and histamine levels — and how to tailor your interventions accordingly.

You’ll learn:

The five neurotransmitter classes – and which genes and cofactors influence each.
The biopterin (BH4) pathway – why it’s essential for dopamine, serotonin, nitric oxide, and how oxidative stress can shut it down.
How inflammation and infections steal tryptophan – driving low serotonin via the kynurenine pathway.
COMT and MAO variants – how fast vs. slow activity changes neurotransmitter levels, mood, and behaviour.
Peroxynitrite, NMDA receptor activity, and neuroinflammation – and how to lower excitotoxicity in the brain.
Genetic and environmental “double hits” – understanding why mood can change suddenly after illness, mould, Lyme, or trauma.
Clinical symptom patterns – how to recognise low vs. high dopamine or serotonin presentations without a genetic test.

Clinical Takeaways:

Identify genetic susceptibilities in MAO and COMT — and match nutrient and herbal strategies to speed up or slow down neurotransmitter clearance.
Support BH4 recycling and reduce oxidative stress to improve neurotransmitter synthesis.
Use lab markers and symptom patterns to distinguish inflammation-driven mood changes from genetically mediated imbalances.
Integrate methylation, detoxification, and neurotransmitter support into a cohesive patient plan.

Perfect for you if:
You want to link your knowledge of methylation and detox pathways to practical strategies for improving mood, cognition, and neurological health — especially in patients with chronic illness, high stress, or genetic vulnerabilities.

Who This Is For

This series is designed for:

Naturopaths, nutritionists, integrative GPs, and other functional health practitioners.
Clinicians wanting to deepen their understanding of biochemical pathways and SNP interpretation.
Practitioners looking for practical, patient-ready strategies that integrate genetic, biochemical, and lifestyle data.

By the End of the Series, You’ll Be Able To:

Map a patient’s folate, methionine, CBS, and neurotransmitter pathways.
Recognise genetic susceptibilities and environmental triggers.
Choose the right nutrient forms and dosing for different genetic profiles.
Troubleshoot “treatment reactions” by identifying upstream or downstream blocks.
Integrate genetics into case taking, lab interpretation, and treatment planning.

Why Practitioners Love This Series

Step-by-step learning — Complex pathways broken down into clear, logical steps.
Clinically applied — Every concept tied to patient presentations and practical interventions.
Integrative approach — Genetics, biochemistry, nutrition, and environmental factors all considered.

Bundle Access Includes:

3 in-depth webinar recordings (watch anytime).
Downloadable pathway diagrams and slides.
Lifetime access to the content for ongoing reference.

Special Bundle Price: $297 (Normally $591 if purchased individually)
Format: On-demand video + downloadable resources
Access: Immediate after purchase

Buy the Complete 3-Part Series Now